PCR, QPCR, AND RT-PCR 🎗️
“𝓘 𝓭𝓸𝓷’𝓽 𝓯𝓲𝓷𝓭 𝓲𝓽 𝓯𝓻𝓾𝓲𝓽𝓯𝓾𝓵 𝓽𝓸 𝓽𝓻𝔂 𝓽𝓸 𝓬𝓸𝓷𝓿𝓲𝓷𝓬𝓮 𝓼𝓸𝓶𝓮𝓸𝓷𝓮 𝓽𝓸 𝓬𝓪𝓻𝓮 𝓪𝓫𝓸𝓾𝓽 𝔀𝓱𝓪𝓽 𝓘 𝓬𝓪𝓻𝓮 𝓪𝓫𝓸𝓾𝓽.” - Dr. Cassandra Extavour 🧬 Polymerase Chain Reaction (PCR), quantitative PCR (qPCR), and reverse transcription PCR (RT-PCR) are closely related molecular techniques used to amplify & analyze nucleic acids. They are fundamental tools in genetics, diagnostics, forensic science, & biomedical research. While they share the core principle of nucleic acid amplification, each technique differs in purpose, input material, & level of quantification. 🔹 The basic principle of PCR is the exponential amplification of a specific DNA segment using repeated thermal cycling. It involves three main steps: denaturation, where double-stranded DNA is separated; annealing, where primers bind to complementary sequences; & extension, where a DNA polymerase synthesizes new strands. Through repeated cycles, a si...
